Noireaux Lab
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Kruyer, N.S., Sugianto, S., Tickman, B.I., Alba Burbano, D., Noireaux, Carothers, J.M., Peralta-Yahya, P. Membrane augmented cell-free systems: a new frontier in biotechnology. ACS Synthetic Biology (2021). paper 

Greiss, F., Daube, S.S., Noireaux, V., Bar-Ziv, R.H. From deterministic to fuzzy decision-making in artificial cells. Nature Communications 11(1), 5648 (2020). paper-SI 

Yim, S.S., Johns, N.I., Noireaux, V., Wang, H.H. Protecting linear DNA templates in cell-free expression systems from diverse bacteria. ACS Synthetic Biology 9, 2851-2855 (2020). paper-SI 

Garenne, D., Noireaux, V. Membrane functions genetically programmed in synthetic cells: a barrier to conquer. Current Opinion in Molecular Systems Biology 24, 9-17 (2020). paper-SI 

Garenne, D., Noireaux, V. Analysis of cytoplasmic and membrane molecular crowding in genetically programmed synthetic cells. Biomacromolecules 21(7), 2808-2817 (2020). paper-SI 

Vonshak, O., Divon, Y., Forste, S., Garenne, D., Noireaux, V., Lipowski, R., Rudorf, S., Daube, S.S., Bar-Ziv, R.H. Programming multi-protein assembly by gene brush patterns and two-dimensional compartment geometry. Nature Nanotechnology 15(9), 783-791 (2020). paper-SI 

Noireaux, V., Liu, A.P. The new age of cell-free biology. Annual Review of Biomedical Engineering 22, 51-77 (2020). paper-SI 

Garenne, D., Libchaber, A., Noireaux, V. Membrane molecular crowding enhances MreB polymerization to shape synthetic cells from spheres to rods. Proc. Nat. Acad. Sci. USA 117, 1902-1909 (2020). paper-SI 

Wandera, K.G., Collins, S.P., Wimmer, F., Marshall, R., Noireaux, V., Beisel, C.L. An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system. Methods 172, 42-59 (2020). paper-SI 

Marshall, R., Beisel, C.L., Noireaux, V. Rapid Testing of CRISPR Nucleases and Guide RNAs in an E. coli Cell-Free Transcription-Translation System. STAR Protocols 1 (2019). paper-SI 

Agrawal, D.K., Marshall, R., Noireaux, V., Sontag, E.D. In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller. Nature Communications 10, 5760 (2019). paper-SI 

Yim, S.S., Johns, N.I., Park, J., Gomes, A.L., McBee, R.M., Richardson, M., Ronda, C., Chen, S.P., Garenne, D., Noireaux, V., Wang, H.H. Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Molecular Systems Biology 15, e8875 (2019). paper-SI 

Marshall, R., Noireaux, V. Quantitative modeling of transcription and translation of an all-E. coli cell-free system. Scientific Reports 9, 11980 (2019). paper-SI 

Garamella, J., Majumder, S., Liu, A.P, Noireaux, V. An Adaptive Synthetic Cell Based on Mechanosensing, Biosensing, and Inducible Gene Circuits. ACS Synthetic Biology 8, 1913-1920 (2019). paper-SI 

Izri, Z., Garenne, D., Noireaux, V., Maeda, Y.T. Gene Expression in on-Chip Membrane-Bound Artificial Cells. ACS Synthetic Biology 8, 1705-1712 (2019). paper-SI 

Garenne, D., Beisel, C.L., Noireaux, V. Characterization of the all-E. coli transcription-translation system myTXTL by mass spectrometry. Rapid Communication in Mass Spectrometry 33, 1036-1048 (2019). paper-SI 

Garamella, J., Garenne, D., Noireaux, V. TXTL-based approach to synthetic cells. Methods in Enzymology 617, 217-239 (2019). paper-SI 

Westbrook, A., Tang, X., Marshall, R., Maxwell, C.S., Chappell, J., Agrawal, D.K., Dunlop, M.J., Noireaux, V., Beisel, C.L., Lucks, J., Franco, E. Distinct timescales of RNA regulators enable the construction of a genetic pulse generator. Biotechnology and Bioengineering 116, 1139-1151 (2019). paper-SI 

Garenne, D., Noireaux, V. Cell-free transcription-translation: engineering biology from the nanometer to the millimeter scale. Current Opinion in Biotechnology 58, 19-27 (2019). paper-SI 

Condon, A., Kirchner, H., Lariviere, D., Marshall, W., Noireaux, V., Tlusty, T., Fourmentin, E. Will biologists become computer scientists? EMBO Reports 19, e46628 (2018). paper-SI 

Kechkeche, D., Cao, E., Grazon, C., Caschera, F., Noireaux, V., Baron Niel, M.L., Dubertret, B. Semiconductor Nanoplatelets: A New Class of Ultrabright Fluorescent Probes for Cytometric and Imaging Applications. ACS Applied Materials and Interfaces 10, 24739-24749 (2018). paper-SI 

Marshall, R., Noireaux, V. Synthetic Biology with an All E. coli TXTL System: Quantitative Characterization of Regulatory Elements and Gene Circuits. Methods in Molecular Biology 1772, 61-93 (2018). paper-SI 

Sakamoto, R., Noireaux, V., Maeda, Y.T. Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions. Scientific Reports 8, 7364 (2018). paper-SI 

Agrawal, D.K., Tang, X., Westbrook, A., Marshall, R., Maxwell, C.S., Lucks, J., Noireaux, V., Beisel, C.L., Dunlop, M.J, Franco, E. Mathematical Modeling of RNA-Based Architectures for Closed Loop Control of Gene Expression. ACS Synthetic Biology 18, 1219-1228 (2018). paper-SI 

Marshall, R., Garamella, R., Noireaux, V., Pierson, A. High-Throughput microliter-sized cell-free transcription-translation reactions for synthetic biology applications using the Echo 550 liquid handler. Labcyte Application Note APP-G124 (2018). paper-SI 

Maxwell, C.S., Jacobsen, Marshall, R., Noireaux, V., Beisel, B.L. A detailed cell-free transcription-translation-based assay to decipher CRISPR protospacer-adjacent motifs. Methods (2018). paper-SI 

Marshall, R., Maxwell, C.S., Collins, S.P., Jacobsen, T., Luo, M.L., Begemann, M.B., Gray, B.N., January, E., Singer, A., He, Y., Beisel, B.L., Noireaux, V. Rapid and scalable characterization of CRISPR technologies using an E. coli cell-free transcription-translation system. Mol. Cell 69, 146-157 (2018). paper-SI 

Klocke, M., Garamella, J., Noireaux, V., Franco, E. Engineering DNA nanotubes for resilience in an E. coli TXTL system. Synthetic Biology 3(1) ysy001 (2018). paper-SI 

Rustad, M., Eastlund, A., Jardine, P., Noireaux, V. Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction. Synthetic Biology 3(1) ysy002 (2018). paper-SI 

Poolman, B., Noireaux, V. Synthetic Biology: from engineering living systems to the bottom-up construction of synthetic Cells. Mighty Microbes, the amazing world of microorganisms ISBN 978-90-816644-4-8 (2017). paper 

Liu, A.P., Noireaux, V. Synthetic cells have senses too. Research Features Issue 120, ISSN 2399-1534 (2017). paper 

Tayar, A., Karzbrun, E., Noireaux, V., Bar-Ziv, R. Synchrony and pattern formation of coupled genetic oscillators on a chip of artificial cells. Proc. Nat. Acad. Sci. USA 114(44) 11609-11614 (2017). paper-SI 

Rustad, M., Eastlund, A., Jardine, P., Noireaux, V. Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System. J Vis Exp (126) (2017). paper-SI 

Dervaux, J., Noireaux, V., Libchaber, A.J. Growth and Instability of a Phospholipid Vesicule in a Bath of Fatty Acids. European Physical Journal Plus 132: 284 (2017). paper-SI 

Majumder, S., Garamella, J., Noireaux, V., Liu, A.P. Cell-sized Mechanosensitive and Biosensing Compartment Programmed with DNA. ChemComm 53, 7349-7352 (2017). paper-SI 

Marshall, R., Maxwell, C.S., Collins, S.P., Beisel, C.L., Noireaux, V. Short DNA Containing Chi Sites Enhances DNA Stability and Gene Expression in E. coli Cell-free Transcription-Translation Systems. Biotechnology and Bioengineering 114(9), 2137-2141 (2017). paper-SI 

Noireaux and others. Cell-free Molecular Dissection. Cell Systems (2017). voices 

Schlesinger, O., Chemla, Y., Heltberg, M., Ozer, E., Marshall, R., Noireaux, V., Jensen, M.H., Alfonta, L. Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids. ACS Synthetic Biology 6, 1076-1085 (2017). paper-SI 

Schenkelberger, M, Shanak, S., Finkler, M., Worst, E.G., Noireaux, V., Helms, V., Ott, A. Expression regulation by a methyl-CpG binding domain in an E. coli based, cell-free TX-TL system. Phys. Biol. 14, 026002 (2017). paper-SI 

Jewett, M.C., Noireaux, V. Synthetic Biology: Tailor-Made Genetic Code. Nature Chemistry 8(40), 291-292 (2016). paper 

Garamella, J., Marshall, R., Rustad, M., Noireaux, V. The all E. coli cell-free TX-TL toolbox 2.0: a platform for cell-free synthetic biology. ACS Synthetic Biology doi: 10.1021/acssynbio.5b00296 (2016). paper-SI 

Caschera, F., Noireaux, V. Compartmentalization of an all E. coli cell-free expression system for the construction of a minimal cell. Artificial Life 22, 1-11 (2016). paper 

Worst, E.G., Exner, M.P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N., Ott, A. Residue-specific incorporation of noncanonical amino acids into model proteins using an Escherichia coli cell-free transcription-translation system. J Vis Exp (114), e54273, doi:10.3791/54273 (2016). paper 
Noireaux, V. Construction de cellules synthetiques: du fondamental au pratique. Medecine/Sciences 31(12), 1126-1132 (2015). paper 

Karzbrun, E, Tayar, A., Noireaux, V., Bar-Ziv, B.H. Propagating gene expression fronts in a 1D coupled system of artificial cells. Nature Physics doi: 10.1038/NPHYS3469 (2015). paper-SI 

Worst, E.G., Exner, M.P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N., Ott, A. Cell-free expression with the toxic amino acid canavanine. Bioorganic & Medical Chemistry Letters 1, 25(17), 3658-60 (2015). paper  supp

Takahashi, M., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, M.M., Noireaux, V., Lucks, J. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions. Methods doi: 10.1016/j.ymeth.2015.05.020 (2015). paper  supp

Chemla, Y,, Ozer, E., Schlesinger, O., Noireaux, V., Alfonta, L. Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system. Biotechnology and Bioengineering doi: 10.1002/bit.25587 (2015). paper  supp

Caschera, F., Noireaux, V. Preparation of amino acid mixtures for cell-free expression systems. Biotechniques 58, 40-43 (2015). paper  supp

Caschera, F., Noireaux, V. A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system. Metabolic Engineering 27, 29-37 (2015). paper  supp

Takahashi, M., Chappell, J., Hayes, C.A., Sun, Z.Z., Singhal, V., Spring, K., Al-Khabouri, S., Fall, C., Noireaux, V., Murray, M.M., Lucks, J. Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems. ACS Synthetic Biology 4(5), 503-515 (2015). paper  supp

Caschera, F., Noireaux, V. Integration of biological parts towards the synthesis of a minimal cell. Current Opinion in Chemical biology 22, 85-91 (2014). paper 

Karzbrun, E, Tayar, A., Noireaux, V., Bar-Ziv, B.H. Programmable on-chip DNA compartments as artificial cells. Science 345(6198), 829-832 (2014). paper  supp

Siegal-Gaskins, D., Tuza, Z., Kim, J., Noireaux, V., Murray, M.M. Resource usage and gene circuit performance characterization in a cell-free ‘breadboard‘. ACS Synthetic Biology 3(6), 416-425 (2014). paper  supp

Coutable, A., Thibault, C., Chalmeau, J., Francois, J.M., Vieu, C., Noireaux, V., Trevisiol, E. Preparation of tethered-lipid bilayers on gold surfaces for the incorporation of integral membrane proteins synthesized by cell-free expression. Langmuir 30(11), 3132-3141 (2014). paper  supp

Coutable, A., Randrianjatovo, I., Noireaux, V., Vieu, C., Thibault, C., Trevisiol, E., Francois, J.M. A comparative study of α-Hemolysin expression in supported lipid bilayers of synthetic and enriched complex bacterial lipid. BioNanoScience 4(2), 104-110 (2014). paper  supp

Sun, Z.Z., Yeung, E., Hayes, C.A., Noireaux, V., Murray, M.M. Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system. ACS Synthetic Biology 3(6), 387-397 (2014). paper  supp

Caschera, F., Noireaux, V. Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription-translation system. Biochimie 99, 162-168 (2013). paper  supp

Siegal-Gaskins, D., Noireaux, V., Murray, R.M. Biomolecular resource utilization in elementary cell-free gene circuits. The Proceedings of the IEEE American Control Conference (2013).

Sun, Z.Z., Hayes, C.A., Shin, J., Caschera, F., Murray, M.M., Noireaux, V. Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology. J Vis Exp (79), e50762 (2013). paper  supp

Shin, J., Jardine, P., Noireaux, V. Genome replication, synthesis and assembly of the bacteriophage T7 in a single cell-free reaction. ACS Synthetic Biology 1(9), 408-413 (2012). paper  supp

Maeda, Y., Nakadai, T., Shin, J., Uryu, K., Noireaux, V., Libchaber, A. Assembly of MreB filaments on vesicular membranes: a synthetic biology approach. ACS Synthetic Biology 1(2), 53-59 (2012). paper  supp

Shin, J., Noireaux, V. An E. coli cell-free expression toolbox: application to synthetic gene circuits and synthetic cell. ACS Synthetic Biology 1(1), 29-41 (2012). paper  supp

Noireaux, V., Maeda, Y., Libchaber, L. Development of an artificial cell, from self-organization to computation and self-reproduction. Proc. Nat. Acad. Sci. USA 108(9), 3473-3480 (2011). paper

Karzbrun, E., Shin, J., Bar-Ziv, R., Noireaux, V. Coarse-grained dynamics of protein synthesis in a cell-free system. Phys. Rev. Letters 106(4), 048104 (2011). paper  supp

Chalmeau, J., Monina, N., Shin, J., Vieu, C., Noireaux, V. Alpha-hemolysin pore formation into a supported phospholipid bilayer using cell-free expression. Biochim Biophys ActA 1808, 271-278 (2011). paper  supp

Shin, J., Noireaux, V. Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system. J. Biol. Eng. 4:9 (2010). paper  supp

Shin, J., Noireaux, V. Efficient cell-free expression with the endogenous E. Coli RNA polymerase and sigma factor 70. J. Biol. Eng. 4:8 (2010). paper  supp

Noireaux, V., Bar-Ziv, R., Godefroy, J., Salman, S., Libchaber, A. Toward an artificial cell based on gene expression in vesicles. Phys. Biol. 2, 1-8 (2005). paper  supp

Noireaux, V., Libchaber, A. A vesicle bioreactor as a step toward an artificial cell assembly. Proc. Nat. Acad. Sci. USA 101, 17669-17674 (2004). paper

Noireaux, V., Bar-Ziv, R., Libchaber, A. Principles of cell-free genetic circuit assembly. Proc. Nat. Acad. Sci. USA 100, 12672-12677 (2003). paper

Boukellal, H., Plastino, J., Noireaux, V., Sykes, C. Propelling soft objects. CR PHYS 4, 275-280 (2003). paper

Dubertret, B., Skourides, P., Norris, D.J., Noireaux, V., Brivanlou, A.H., Libchaber, A. In vivo imaging of quantum dots encapsulated in phospholipids micelles. Science 298, 1759-1762 (2002). paper  supp

Fradelizi, J., Noireaux, V., Plastino, J., Menichi, B., Louvard, D., Sykes, C., Golsteyn, R.M., Friederich, E. ActA and the human Zyxin harbour Arp2/3 complex independent actin polymerisation activity. Nat. Cell Biol. 3, 699-707 (2001). paper

Noireaux, V., Golsteyn, R.M., Friederich, E., Prost, J., Antony, C., Louvard, D., Sykes, C. Growing an actin gel on spherical surfaces. Biophy. J. 78, 1643-1654 (2000). paper

Plastino, J., Noireaux, V., Louvard, D., Antony, C., Sykes, C., Golsteyn, R.M. Dynamic cellular actin polymerization in vitro. Recent Res. Devel. Biophys. Chem. 1, 15-25 (2000).

Gerbal, F., Noireaux, V., Sykes, C., Julicher, F., Chaikin, P., Ott, A., Prost, J., Golsteyn, R.M., Freiderich, E., Louvard, D., Laurent, V., Carlier, M.F. On the Listeria propulsion mechanism. PRAMANA-J.Phys. 53, 155-170 (1999). paper

Noireaux publications on Google Scholar

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